Materials and methods Sera of 78 patients with APS type 2, 3, 4 and 109 healthy subjects were screened for HLA II genes' polymorphism. APS patients were at the age of 18-78 years, women-74,4%, men-25,6%. The control healthy group was at the age of 18-58, among them women-64,2 %, men-35,8 %/ The difference between two groups was not statistically significant on gender (р=0,154). Molecular-genetic examination of HLA II genes was conducted for all patients. DNA from the whole blood of the person

more »

... s carried out by set of QIAamp DNA Blood Mini Kit (QIAGEN). The method of multyprimer allele-specified polymerase chain reaction was used. Statistical analysis was carried out by STATISTICA 10, SPSS Statistics with the use of precise two-sided Fisher's test. The differences Endocrinol Metab Int J. 2018;6(6):434-438. 434 Abstract Introduction: The aim of the study was to determine the association of chronic adrenal insufficiency with polymorphism of HLA II genes among patients with APS 2,3,4 types. The focus of the study was on the revealing of protective genes for Addison's disease in APS 3 type patients. Materials and methods: The case-control study involved 78 patients with APS 2, 3, 4 types and 109 healthy subjects. Alleles of the HLA II class genes, CTLA4 and PTPN22 were identified by the multiprimer allele-specific PCR method. The statistical analysis was carried out using the exact two-sided Fisher test. The association of the chronic adrenal insufficiency in patients with APS was determined by the value of the odds ratio (OR -odd's ratio), the value of 95% confidence interval (95% CI).