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Immune synapse instructs epigenomic and transcriptomic functional reprogramming in dendritic cells

Hospital Universitario La Princesa, Universidad Autónoma de Madrid, 28006 Madrid, Spain., Centro Nacional de Investigaciones Cardiovasculares (CNIC), 28029 Madrid, Spain., University of Fribourg, 1700 Fribourg, Switzerland., Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBERCV), 28029 Spain., Ana Alcaraz-Serna, Eugenio Bustos-Morán, Irene Fernández-Delgado, Francisco Sánchez-Madrid, Ana Alcaraz-Serna, Eugenio Bustos-Morán, Irene Fernández-Delgado, Diego Calzada-Fraile (+13 others)
2021 IBJ Plus  

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Understanding the fate of dendritic cells (DCs) after productive immune synapses (postsynaptic DCs) with T cells during antigen presentation has been largely neglected in favor of deciphering the nuances of T cell activation and memory generation. Here, we describe that postsynaptic DCs switch their transcriptomic signature, correlating with epigenomic changes including DNA accessibility and histone methylation. We focus on the chemokine receptor Ccr7 as a proof-of-concept gene that is
more » ... in postsynaptic DCs. Consistent with our epigenomic observations, postsynaptic DCs migrate more efficiently toward CCL19 in vitro and display enhanced homing to draining lymph nodes in vivo. This work describes a previously unknown DC population whose transcriptomics, epigenomics, and migratory capacity change in response to their cognate contact with T cells.
doi:10.24217/2531-0151.21v1s4.00035 fatcat:cpce5sgqhzcfrb47hhdrfqx7gm
Citation

Hospital Universitario La Princesa, Universidad Autónoma de Madrid, 28006 Madrid, Spain., Centro Nacional de Investigaciones Cardiovasculares (CNIC), 28029 Madrid, Spain., University of Fribourg, 1700 Fribourg, Switzerland., Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBERCV), 28029 Spain., Ana Alcaraz-Serna, Eugenio Bustos-Morán, Irene Fernández-Delgado, Francisco Sánchez-Madrid, Ana Alcaraz-Serna, Eugenio Bustos-Morán, Irene Fernández-Delgado, Diego Calzada-Fraile, Daniel Torralba, Ester Marina-Zárate, Erika Lorenzo-Vivas, Enrique Vázquez, Manuel José Gómez, Fátima Sánchez-Cabo, Ana Dopazo, Almudena Ramiro, Francisco Sánchez-Madrid, Juliana Barreto de Alburquerque, Nora Ruef, Jens V Stein, Francisco Sánchez-Madrid. "Immune synapse instructs epigenomic and transcriptomic functional reprogramming in dendritic cells." IBJ Plus (2021)