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Selection of yeast auxotrophs by thymidylate starvation
1977
Journal of Bacteriology
Preserved Fulltext
A rapid procedure for the recovery of Saccharomyces ceret'isiae auxotrophs was developed by exploiting the protection of these mutants from thymineless death when a required metabolite was withheld. The method can be used for thymidine 5'-monophosphate-requiring auxotrophs or wild-type strains blocked in de novo synthesis of thymidylate by folate antagonists.
doi:10.1128/jb.132.3.1036-1037.1977
fatcat:6cqr6r4sjbcbhlcn6khj4fqziu