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Slow-phase Kinetics of Nucleotide Binding to the Uncoupling Protein from Brown Adipose Tissue Mitochondria
1998
Journal of Biological Chemistry
The kinetics of nucleotide binding to the uncoupling protein (UCP) from brown adipose tissue mitochondria were studied with a filter binding method. Fast and slow phases of binding were observed, corresponding to the two-stage binding model based on equilibrium binding studies (Huang, S. G., and Klingenberg, M. (1996) Biochemistry 35, 7846 -7854) (Reaction 1). Although this method determines total binding, only the slow phase can be resolved. The fast unresolved phase represents the formation
doi:10.1074/jbc.273.2.859
pmid:9422742
fatcat:kyjnnr6zpjfvted5c6fjpxm4ha