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Post-fixation of sections is especially required for cryostat sections of fresh frozen tissues. Vimentin is an intermediate filament in both fibrillary and non-fibrillary form, expressed in Müller's cells and astrocytes of the retina. Our aim was to determine the best post-fixation method for visualising vimentin in archival mouse eyes. Materials and methods: We used an archival mouse eye, slightly pre-fixed with paraformaldehyde and stored at -80°C for 4 years. We tried three fixativesdoi:10.5603/fm.a2017.0082 pmid:28868606 fatcat:tlzddpl75vfrrihh3uxf3gsy5a