Extension of the transcriptional and translational map of the left end of the vaccinia virus genome to 21 kilobase pairs

J A Cooper, R Wittek, B Moss
1981 Journal of Virology  
Physical, transcriptional, and translational maps of an EcoRI fragment located between 15,800 and 20,600 base pairs from the left end of the vaccinia virus genome were prepared. Major polypeptides with molecular weights of 14,000 (14K polypeptide), 32,000 and 38,000 were synthesized in a reticulocyte cell-free system programed with immediate early RNA made in the presence of cycloheximide and selected by hybridization to X recombinant DNA containing the EcoRI Virol. 28:171-181. 25. Wittek, R.,
more » ... 1. 25. Wittek, R., and B. Moss. 1980. Tandem repeats within the inverted tenninal repetition of vaccinia virus DNA.
doi:10.1128/jvi.39.3.733-745.1981 fatcat:od77pkiqc5cfppskrdmq3jmhfq