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Cooperative Conformational Changes in a G-protein-coupled Receptor Dimer, the Leukotriene B4Receptor BLT1
Journal of Biological Chemistry
We have used an isolated receptor, the leukotriene B 4 receptor BLT1, to analyze the mechanism of receptor activation in a G-protein-coupled receptor dimer. The isolated receptor is essentially a dimer whether the agonist is present or not, provided the detergent used stabilizes the inactive dimeric assembly. We have produced a receptor mutant where Cys 97 in the third transmembrane domain has been replaced by a serine. This mutation leads to an ϳ100-fold decrease in the affinity for thedoi:10.1074/jbc.m404941200 pmid:15358776 fatcat:w2ru33wlpfdqrmnt25w6r2nj7i