Analysis of vertebrate SCL loci identifies conserved enhancers

Berthold Göttgens, Linda M. Barton, James G.R. Gilbert, Anthony J. Bench, Maria-José Sanchez, Sabine Bahn, Shailesh Mistry, Darren Grafham, Amanda McMurray, Mark Vaudin, Enrique Amaya, David R. Bentley (+1 others)
<span title="">2000</span> <i title="Springer Nature"> <a target="_blank" rel="noopener" href="" style="color: black;">Nature Biotechnology</a> </i> &nbsp;
The SCL gene encodes a highly conserved bHLH transcription factor with a pivotal role in hemopoiesis and vasculogenesis. We have sequenced and analyzed 320 kb of genomic DNA composing the SCL loci from human, mouse, and chicken. Long-range sequence comparisons demonstrated multiple peaks of human/mouse homology, a subset of which corresponded precisely with known SCL enhancers. Comparisons between mammalian and chicken sequences identified some, but not all, SCL enhancers. Moreover, one peak of
more &raquo; ... human/mouse homology (+23 region), which did not correspond to a known enhancer, showed significant homology to an analogous region of the chicken SCL locus. A transgenic Xenopus reporter assay was established and demonstrated that the +23 region contained a new neural enhancer. This combination of long-range comparative sequence analysis with a high-throughput transgenic bioassay provides a powerful strategy for identifying and characterizing developmentally important enhancers.
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="">doi:10.1038/72635</a> <a target="_blank" rel="external noopener" href="">pmid:10657125</a> <a target="_blank" rel="external noopener" href="">fatcat:gb3hntcsrfgffnkokggvdenr7a</a> </span>
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