Role of adiponectin in adipose tissue wound healing

C.E. Jin, L. Xiao, Z.H. Ge, X.B. Zhan, H.X. Zhou
2015 Genetics and Molecular Research  
The purpose of this study was to investigate the mechanism behind adipose tissue wound healing (ATWH). The preadipocyte cell line 3T3-L1 was cultured and expression of adiponectin receptors (AdipoR1/2) was detected by immunohistochemistry and reverse transcription polymerase chain reaction. The concentration of adiponectin secreted at different cell densities was measured by enzyme-linked immunosorbent assay, while preadipocyte proliferation and migration were determined in vitro by MTT and
more » ... itro by MTT and wound closure assays. AdipoR1/2 were found to be expressed in 3T3-L1 preadipocytes. There were no statistically significant differences in the concentrations of adiponectin secreted by cell solutions of different densities (P > 0.05). In addition, adiponectin was seen to promote the growth and migration of preadipocytes. In conclusion, adiponectin may regulate ATWH by promoting preadipocyte proliferation and migration, and its systemic and/or local application is proposed as a 8884 C.E. Jin et al. ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 8883-8891 (2015) promising therapeutic approach for the treatment of wounds incurred as a result of surgery.
doi:10.4238/2015.august.3.11 pmid:26345819 fatcat:vzguckjch5hl7azfuc6uyswe74