Behavioral changes of migratory species have been globally documented in recent decades. However, there is a paucity of research on changes in migratory bat species. Brazilian free-tailed bats (Tadarida brasiliensis) roost in central Texas from March to November. These bats have historically migrated south in late fall, leaving summer roosts unoccupied during winter. Recently, overwintering populations have been discovered in central Texas. The objectives of our study were to determine presence

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... or absence of overwintering free-tailed bats at six known summer roosts, obtain baseline population estimates, and evaluate microclimates of roosts during winters of 2010-2011 and 2011-2012. We used data loggers to monitor temperature and humidity hourly. We estimated population sizes with digital images using ImageJ software, previously established roosting densities, or both. Our results indicated that occupied roosts were colder, had less stable temperatures, and had a stronger correlation between internal and external temperatures vs. unoccupied roosts. Population sizes increased at all occupied roosts from 2010