We performed SDS-PAGE to search for a connectin-like proteinin echinoderm sea urchins. We found a band that had high molecular weight, and predicted that it might be a connectin-like protein. In addition, we performed RT-PCR based on the sea urchin genome sequence, and determined the sequence of a connectin-like protein. Its size was about 80 kbp, and its molecular weight was estimated to be about 3 million. We noticed that this protein contains both connectin-like and characteristic structures

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... from tandem immunoglobulin domains, a PEVK region, and a tandem fibronectin type 3 domain. Additionally, we prepared an antibody based on its sequence, and performed western blotting. We found that this antibody showed a reaction to the high molecular weight band. 2P140 分子動力学シミュレーションを用いたトロポミオシンの柔軟 性および屈曲性の解析 Analysis of flexibility and curvature of tropomyosin by molecular dynamics simulation Tropomyosin (TM) is a dimeric parallel-coiled-coil protein, which binds the actin filament and participates in the regulation of muscle contraction. In addition, TM plays a role in the actin assembly in non-muscle cells. It is likely that the flexibility and curvature of TM affect these functions, through the interaction with actin. In order to evaluate these mechanical properties in detail, we performed molecular dynamics simulation with implicit solvent (by amber 10) for rabbit full-length TM. The crystal structure of pig TM (PDB ID 1C1G) was used as an initial structure, and the amino acids were replaced by rabbit TM sequence. In this study, the highly flexible and curved residues were determined and the relevance to the actin binding will be discussed. 2P141 横紋筋筋原線維 SPOC の動的特性に関するモデルシミュレー ション Model simulation on the dynamic properties of SPOC in a striated myofibril