Nano Aptasensor for Protective Antigen Toxin of Anthrax

Lakshmi N. Cella, Pablo Sanchez, Wenwan Zhong, Nosang V. Myung, Wilfred Chen, Ashok Mulchandani
2010 Analytical Chemistry  
We demonstrate a highly sensitive nano aptasensor for anthrax toxin through the detection of its polypeptide entity, protective antigen (PA toxin) using a PA toxin ssDNA aptamer functionalized single-walled carbon nanotubes (SWNTs) device. The aptamer was developed inhouse by capillary electrophoresis systematic evolution of ligands by exponential enrichment (CE-SELEX) and had a dissociation constant (K d ) of 112 nM. The aptasensor displayed a wide dynamic range spanning up to 800 nM with a
more » ... ection limit of 1 nM. The sensitivity was 0.11 per nM, and it was reusable six times. The aptasensor was also highly selective for PA toxin with no interference from human and bovine serum albumin, demonstrating it as a potential tool for rapid and point-of-care diagnosis for anthrax. Omenaca, C.; Topiel, M. S.; Galbraith, M.; Tapper, M.; Fisk, T. L.; Zaki, S.; Popovic, T.; Meyer, R. F.; Quinn, C. P.; Harper, S. A.; Fridkin, S. K.; Sejvar, J. J.; Shepard, C. W.; McConnell, M.; Guarner, J.; Shieh, W. J.; Malecki, J. M.; Gerberding, J. L.; Hughes, J. M.; Perkins, B. A. Emerging Infect. Dis. 2001, 7 (6), 933-944.
doi:10.1021/ac902791q pmid:20136122 pmcid:PMC2930939 fatcat:erquslenurd6pbi5mlvteqkd7e