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Earliest cells of neural lineage express pluripotency and early neural genes. However, conditions essential for propagating these cells are not known. In this study, we investigated several factors that would support and propagate embryonic stem cells (ES) derived early neural stem cells. RT-qPCR data revealed that PD325901 (MEK inhibitor), Leukemia inhibitory factor (LIF) and KN93 (CAMKII inhibitor) (henceforth referred as PLK media) upregulated expression of early neural genes Sox1 and Pax6doi:10.21203/rs.3.rs-1699524/v1 fatcat:ij3ltgzqdnaqpocssgazh26hnm