Seven halogenated hydroxy diphenyl ether derivatives have been synthesized, 6 of them are new compounds and their structures were identified by 1 H NMR, HR-MS and IR spectra. The antibacterial activities of all the present compounds were tested via the agar-well diffusion method in vitro in the concentration of 50 µg/mL. The results showed that most of the new compounds exhibited antibacterial activities against the tested bacterium.