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The simultaneous wing movement by multiple worker bees in a colony produces a hissing sound, which is a novel acoustic and vibrational signal of the honey bees. Hissing of honey bees is thought to be a response to direct, threatening stimuli. However, we discovered Japanese honey bees (Apis cerana japonica ) can hiss even without obvious disturbances in previous study. In this study, to understand the temporal characteristics of honey bee hissing, we conducted 24-h sound recordings over 7doi:10.1007/s13592-018-0631-x fatcat:czp7hqeyubhbxdoq26o6nljmf4