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B cells expressing authentic naive human VRC01-class BCRs can be primed and recruited to germinal centers in multiple independent mouse models
Animal models of human antigen-specific B cell receptors (BCR) generally depend on inferred germline sequences, and thus their relationship to authentic naive human B cell BCR sequences and affinities is unclear. Here, BCR sequences from authentic naive human VRC01-class B cells from healthy human donors were selected for the generation of three new BCR knock-in mice. The BCRs span the physiological range of affinities found in humans, and use three different light chains (VK3-20, VK1-5, anddoi:10.1101/2020.02.24.963629 fatcat:i2u3sm75wvcmlktmhb73bt533u