The Relationship Between MicroRNAs And Congenital Kidney Anomalies

Kenan, Zubeyde, Mehmet Serdar, Ruhan, İsmail, Sibel
2020 Dicle Medical Journal  
Objective: The purpose of this study was to identify diagnostic microRNAs (miRNAs) associated with congenital kidney anomalies. Methods: Twenty-five healthy pregnant women who were found to have fetal kidney anomaly in the second trimester of their pregnancy at Department of Perinatology, were included in the study. Serum samples were taken from the pregnant women at the moment of diagnosis in the antenatal 20th gestational week, whereas serum samples were taken from the cord blood of babies
more » ... ing birth. There were 11 multicystic dysplastic kidney patients, 6 autosomal recessive polycystic kidney patients, and 8 unilateral hypoplastic kidney patients. Expression of specific miRNAs was monitored using specific primer assays in Real-Time PCR. The expression of the following miRNAs was quantified: miR-17, miR-192, miR-194, miR-204, miR-215, and miR-216. Results: mir-17 expression was significantly lower in children with congenital kidney anomalies than in the control group. ROC curve analysis showed that the area under the curve was 0.700 for miR-17 in the prediction of congenital kidney anomalies. Conclusions: In children with congenital kidney anomalies, miR-17 expression was significantly different than in the control group. Thus, this miRNA may be used in the antenatal early detection of these congenital kidney anomalies.
doi:10.5798/dicletip.706048 doaj:dc9954b5ae2c4a7795ed564c1392ae27 fatcat:bhfsrybnqfhdlm75cqiew4gjbq