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Sequencing whole plant genomes has advanced rapidly with the development of next generation sequencing (NGS) technologies and bioinformatics, enabling the study of large and complex genomes such as that of the hexaploid cereal, Triticum aestivum L. (bread wheat). Despite advances, however, confounding factors such as repetitive elements and low polymorphism still hinder sequencing attempts. Isolation techniques such as sequencing of diploid progenitors and chromosome separation through flowdoi:10.4236/as.2017.82014 fatcat:kczpce325rg47mezah4hyurkki