Ассоциативные бактерии к Triticum aestivum L. черноземов южного и обыкновенного

Мельничук Татьяна Николаевна, Еговцева Анна Юрьевна, Абдурашитов Сулейман Февзиевич, Андронов Евгений Евгеньевич, Абдурашитова Эльвина Расимовна, Радченко Александр Федорович, Ганоцкая Татьяна Леонидовна, Радченко Людмила Анатольевна
Currently, a promising area of research is the development of rhizosphere biotechnologies using associative bacteria to a particular plant species, which can increase their potential and quality, which in turn contributes to the stability of agro-ecosystems and preservation of the environment. The aim of the research was to study the wheat rhizosphere microbiome and strains of associative bacteria isolated from different varieties grown on southern and ordinary chernozems. The studies were
more » ... e studies were carried out in 2018 on three winter wheat varieties (Triticum aestivum L.) 'Bagheera', 'Lydia' and 'Ermak'. Modern approaches to the study of the taxonomic structure of the rhizosphere microbiome using high-throughput sequencing of the 16S rRNA gene libraries were used in the process of research. The community of prokaryotes of rhizosphere chernozem mainly consisted of representatives of 10 phyla: Crenarchaeota from the archaea and bacterium domain: Acidobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Firmicutes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia. Representatives of the non-attributable domain of prokaryotes, the share of which in the samples of chernozem was 2.4 - 4.0 %, were identified. Phylum Proteobacteria had the highest proportion in the studied varieties, and the difference in soils was insignificant ('Bagheera' - 25.5 - 27.3 %, 'Ermak' - 24.3 - 29.1 %, 'Lydia' - 23.7 - 26.1 %). Five new strains of associative to wheat bacteria identified by the method of sequencing the 16S rRNA gene belonged to five species from different taxonomic groups and showed different efficacy on southern and ordinary chernozems. Strains of Bacillus sp. B5 and Agrobacterium tumefaciens R1 provided an increase in shoot biomass of the 'Bagheera' variety on ordinary chernozem by 0.10 g (48 %) and 0.13 g (62 %), respectively, compared to the control (0.21 g). Thus, changes in the taxonomic composition of the southern and ordinary chernozems rhizosphere of three wheat varieties had been determined under the in [...]
doi:10.25637/tvan.2018.16.21150 fatcat:nvdscpccszcqxlhg7msipzdkpy