EFFECT OF OINTMENT FROM COMBINED Centella asiatica (L.) Urban AND Coleus amboinicus EXTRACTS ON SKIN WOUND TISSUE REPAIR IN DIABETIC RATS
A single Centella asiatica L. Urban (CALU) extract stimulated cellular proliferation in granulation tissue, while a single Coleus amboinicus L. (CAL) extract acted as antiseptic agent. Diabetic wound closure is sensitively difficult to be done because proliferation is likely inhibited by antiseptic alteration resulted as infectious wound. Both extracts may give synergy in combination into ointment. Objective: to investigate the effect of combined-extracts ointment on angiogenesis, inflammatory
... esis, inflammatory cells and microscopic wound diameter. Methods: posttest only group design was experimentally conducted, sixty rats were randomly divided into 5 groups; each group was treated with different ointment constituent: single 1,5% CALU extract; single 5% CAL extract; both extracts (1,5%CALU+ 5%CAL); placebo and Sanoskin Melladerm Plus® (SMP). Diabetic rat's skin was excised and treated with hydrocarbon-based ointment. Assessment on angiogenesis, inflammatory cells and wound diameter were observed histopathologically on day 3, 7 and 14 after treatment. The data were analyzed by one-way ANOVA and LSD. Results: combined-extracts ointment effect was significantly different (p<0,05) compared to either single CALU, single CAL, placebo or SMP® in term of angiogenesis and inflammatory cells, but not significantly different (p>0,05) compared to SMP® in term of wound diameter reduction. Conclusion: Combined-extracts ointment is better than either single extract, placebo or SMP® in improving angiogenesis and reducing number of inflammatory cells, but not better than SMP® in reducing wound diameter.