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Development of an in vitro cleavage assay system to examine vaccinia virus I7L cysteine proteinase activity
2005
Virology Journal
Through the use of transient expression assays and directed genetics, the vaccinia virus (VV) I7L gene product has been implicated as the major maturational proteinase required for viral core protein cleavage to occur during virion assembly. To confirm this hypothesis and to enable a biochemical examination of the I7L cysteine proteinase, an in vitro cleavage assay was developed. Using extracts of VV infected cells as the source of enzyme, reaction conditions were developed which allowed
doi:10.1186/1743-422x-2-63
pmid:16105175
pmcid:PMC1198262
fatcat:swt73mlibrbepffaapkaee27ci