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s Abstract Antibodies are unique in their high affinity and specificity for a binding partner, a quality that has made them one of the most useful molecules for biotechnology and biomedical applications. The field of antibody engineering has changed rapidly in the past 10 years, fueled by novel technologies for the in vitro isolation of antibodies from combinatorial libraries and their functional expression in bacteria. This review presents an overview of the methods available for the de novodoi:10.1146/annurev.bioeng.2.1.339 pmid:11701516 fatcat:ifkgo3sckzfgdpdcsgg4j5qqze