ESTABLISHMENT OF CALLUS CULTURES OF ANDROGRAPHIS PANICULATA FOR THE ASSESSMENT OF ANDROGRAPHOLIDE CONTENT
International Journal of Research in Ayurveda and Pharmacy
Andrographolide is the main bioactive component of the medicinal plant, Andrographis paniculata. Callus cultures of Andrographis paniculata are known to produce only paniculides (sesquiterpene lactones) and not andrographolides (diterpene lactone) that are produced by the intact plant. In this study, Callus cultures of Andrographis paniculata were established from leaf explant. Different growth regulators greatly influenced the growth of callus cultures. Callus was established on Murashige and
... d on Murashige and Skoog medium at various concentrations of 2, 4-D & TDZ alone (1.0-2.5 mg l -1 ) and in combinations (2, 4-D + NAA, 2, 4-D + Kn, BAP+ NAA). Best callus induction was obtained at MS + 2, 4-D + NAA (1.0 +1.0 mg l -1 ). HPLC of best callus samples were undergone to detect their andrographolide content. The highest amount of andrographolide content (7.64 mg g -1 FW) was observed in callus grown on MS supplemented with 1.0 mg l -1 2, 4-D + 0.5 mg l -1 NAA followed by 1.0 mg l -1 2, 4-D + 1.5 mg l -1 Kn and 2.5 mg l -1 BAP + 0.5 mg l -1 NAA. The present study thus indicates that andrographolide production can be induced in the callus of A. paniculata by treatment with plant growth hormones, although the content predominates in leaves and stem.