Malaria seroprevalence in blood bank donors from endemic and non-endemic areas of Venezuela

Carmen Elena Contreras, Marcos de Donato, María Ana Rivas, Hectorina Rodulfo, Robert Mora, María Eulalia Batista, Norka Marcano
2011 Memórias do Instituto Oswaldo Cruz  
In Venezuela, a total of 363,466 malaria cases were reported between [1999][2000][2001][2002][2003][2004][2005][2006][2007][2008][2009]. Several states are experiencing malaria epidemics, increasing the risk of vector and possibly transfusion transmission. We investigated the risk of transfusion transmission in blood banks from endemic and non-endemic areas of Venezuela by examining blood donations for evidence of malaria infection. For this, commercial kits were used to detect both
more » ... ct both malaria-specific antibodies (all species) and malaria antigen (Plasmodium falciparum only) in samples from Venezuelan blood donors (n = 762). All samples were further studied by microscopy and polymerase chain reaction (PCR). The antibody results showed that P. falciparum-infected patients had a lower sample/cut-off ratio than Plasmodium vivax-infected patients. Conversely, a higher ratio for antigen was observed among all P. falciparum-infected individuals. Sensitivity and specificity were higher for malarial antigens (100 and 99.8%) than for antibodies (82.2 and 97.4%). Antibodypositive donors were observed in Caracas, Ciudad Bolívar, Puerto Ayacucho and Cumaná, with prevalences of 1.02, 1.60, 3.23 and 3.63%, samples were observed among the donors. However, our results show significant levels of seropositivity in blood donors, suggesting that more effective measures are required to ensure that transfusion transmission does not occur.
doi:10.1590/s0074-02762011000200002 pmid:21537669 fatcat:6ylwprcfwvcr5hfdwjkpgjjfbq