MicroRNA‑590‑3p relieves hypoxia/reoxygenation induced cardiomyocytes apoptosis and autophagy by targeting HIF‑1α

Nengjing Gong, Xiaohuai Yang, Xiaoyu Li, Yuyu Jiang, Cuifang Gu, Shasha Ma, Qin Gao, Xiangyang Cheng
2021 Experimental and Therapeutic Medicine  
Autophagy and apoptosis are key factors in myocardial ischemia/reperfusion (I/R) injury. MicroRNAs (miRNAs or miRs) participate in occurrence and development of myocardial I/R injury by regulating autophagy and apoptosis. The purpose of the present study was to investigate the role of miR-590-3p in the regulation of autophagy and apoptosis in hypoxia/reoxygenation (H/R)-treated cardiomyocytes. Following 6 h hypoxia and 6 h reoxygenation in primary rat cardiomyocytes, miR-590-3p was
more » ... . Transfection of miR-590-3p mimic inhibited the increased autophagy and apoptosis following H/R treatment. Subsequent experiments demonstrated that miR-590-3p regulated induction of autophagy and apoptosis by targeting hypoxia inducible factor (HIF)-1α. Forced expression of HIF-1α rescued the protective effect of miR-590-3p on H/R-induced cardiomyocytes. In summary, the present study showed that miR-590-3p exhibited a protective effect on H/R-induced cardiomyocyte injury and may be a novel target for the treatment of myocardial ischemia disease.
doi:10.3892/etm.2021.10511 fatcat:4nzmoq4tszffjoegfxrf6rekia