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Gated acquisition of "T nuclear magnetic resonance spectra from perfused ferret hearts loaded with the fluorinated Ca I+ indicator 5,5'-F 2 -BAPTA allows direct quantitation of the cyclical changes in the intracellular free Ca 2+ concentration ([Ca 2+ ],) that underlie contraction in intact hearts. [Ca 2+ ], increased from approximately 200 nM in diastole to -1 fiM or higher hi early systole. Although the 19 F spectra that report [Ca 2+ ], changed dramatically and reproducibly during thedoi:10.1161/01.res.63.3.673 pmid:3136952 fatcat:sgdjxvi5wfgxvibqvgzp4wjoy4