A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2021; you can also visit the original URL.
The file type is application/pdf
.
SIESTA as a universal unbiased proteomics approach for identification and prioritization of enzyme substrates
[post]
2021
unpublished
This protocol describes the proteomics technique called System-wide Identification and prioritization of Enzyme Substrates by Thermal Analysis or SIESTA 1,2. SIESTA can be used for universal discovery of enzyme substrates that shift in thermal stability or solubility upon post-translational modification (PTM). Experimental design, proteomics sample preparation and data analysis are the key stages of this protocol. Data analysis can be performed using our SIESTA package hosted on GitHub 3. When
doi:10.21203/rs.3.pex-1327/v1
fatcat:vmlz3wlscvfpjaduzsmebo7vlu