1a [alpha], 25-dihydroxyvitamin D3 induced osteogenesis in murine embryonic stem cells: the role of Wnt5a [article]

Lesley Anne Margaret Davis, Derrick Rancourt, University Of Calgary, University Of Calgary
2007
The undersigned certify that they have read, and recommend to the Faculty of Graduate Studies for acceptance, a thesis entitled "1α, 25-dihydroxyvitamin D 3 induced osteogenesis in murine embryonic stem cells: the role of Wnt5a" Abstract This thesis explores the role of Wnt5a during in vitro differentiation of murine embryonic stem cells to osteoblasts. It shows for the first time that Wnt5a gain of function increases mineralization, specifically calcification, during osteoblast
more » ... ast differentiation. This increase in mineralization is dependent on Wnt5a concentration upon osteoinduction. In addition, Wnt5a acts with 1α, 25-dihydroxyvitamin D 3 to increase the expression of bone specific genes, such as alkaline phosphatase, osteopontin and osteocalcin. The increase in alkaline phosphatase and osteopontin expression occurred earlier than previously reported; suggesting that the addition of Wnt5a could accelerate osteogenesis in vitro.
doi:10.11575/prism/22803 fatcat:7bnbxmw5tfa3tp22bmrevhvgee