EFFECT OF INCUBATION TEMPERATURE ON DENGUE-2 VIRAL ANTIGEN PRODUCTION AND VIRAL RNA SYNTHESIS IN INFECTED Aedes albopictus CLONE C6/36 CELL LINE

Marlou Noel M. MANGADA, Halimah MOHAMED, Leticia del Carmen CASTILLO, Futoshi HASEBE, Akira IGARASHI
1995 Tissue Culture Research Communications  
Increase in the incubation temperature of infected Aedes albopictus clone C6/36 cells from its optimal growth temperature of 28°C to 32 and 37°C showed a concomitant rise in the production of dengue virus type 2 antigen and genomic RNA. The quantitative reverse transcription-polymerase chain reaction using an internal standard as a measuring tool for the viral genomic RNA showed that RNA production was higher in the cells and in the extracellular fluid from the cultures incubated at 32 and 37°C
more » ... than at 28°C. The presence of viral antigen and viral genomic RNA was also detected 1-2 days earlier in the cultures incubated at elevated temperatures.
doi:10.11418/jtca1981.14.4_213 fatcat:pwc54zhbsvdn3keseafwrsogda