Estimation of the galanthamine using derivative spectrophotometry in bulk drug and formulation

Karan Mittal, Ramni Kaushal, Rajashree Mashru, Arti Thakkar
2010 Journal of Biomedical Science and Engineering  
Two simple, rapid, accurate, precise, reliable and economical spectrophotometric methods have been proposed for the determination of galanthamine hydrobromide (GH) in bulk and pharmaceutical formulation. First method is zero order UV spectrophotometry and second is 1st derivative zero crossing spectrophotometry. The developed methods have shown best results in terms of linearity, accuracy, precision, LOD and LOQ for bulk drugs and marketed formulations. Absorbance was measured at 287 nm for
more » ... d at 287 nm for zero order and 277.4 nm for first derivative. It obeyed Lambert-Beer's law in the range of 30-80 μg mL-1. Both methods have good linearity (r2 = 0.9997) and accuracy found to be 100.5% and 101.2% for both methods respectively. The working solutions were prepared by accurately diluting aliquots of the standard solution with water to obtain the concentration in range of 30-80 μg mL-1. The absorption spectra of the samples were recorded between 200-400 nm against double distilled water using a 1.0 cm K. Mittal et al. / JBiSE 440 quartz cell. Zero order spectra of pure drug GH were stored individually within mentioned concentration ranges and absorbance was measured at 280 nm.
doi:10.4236/jbise.2010.34060 fatcat:qw44ftjolvgstk7o6e77s3ylnu