Is Urine Interleukin-8 Level a Reliable Laboratory Test for Diagnosing Late Onset Sepsis in Premature Infants?

M. R. Bentlin, L. M. S. de Souza Rugolo, A. R. Junior, M. Hashimoto, J. C. Lyra
2007 Journal of Tropical Pediatrics  
The present study is aimed to determine serum and urine interleukin-8 (IL-8) levels in premature infants with late onset sepsis (LOS) and to evaluate if urine IL-8 is a useful test for LOS diagnosis. Fifty-six premature infants admitted to the NICU over 1 year had serum and urine IL-8 determined by ELISA. They were divided into three groups: I definite sepsis, II probable sepsis and III non-infected. Results were expressed as mean or median. Differences between groups were assessed by ANOVA,
more » ... sessed by ANOVA, Kruskal-Wallis ANOVA and Dunn's Method. Sensitivity, specificity and positive and negative predictive values were calculated and a receiver operator characteristic curve was constructed to determine serum and urine IL-8 accuracy. There were no differences between groups for birth weight, and gestational and post-natal age. Median serum and urine IL-8 levels were significantly higher in GI and GII: 929 Â 906 Â 625 pg/ml; P ¼ 0.024, and 249 Â 189 Â 42 pg/mgCr; P < 0.001. Optimal cut-off point was 625 pg/ml for serum IL-8 with 69% sensitivity and 75 pg/mgCr for urine IL-8 with 92% sensitivity. IL-8 can be determined in urine from premature infants with LOS and is an accurate and feasible diagnosis method. ) NPV, negative predictive value; PPV, positive predictive value. Parentheses indicate 95% CI. M. R. BENTLIN ET AL.
doi:10.1093/tropej/fmm054 pmid:17596292 fatcat:vfuco3r6mrebzf6kyrdcacfw4y