Antibacterial Activity of Ethyl Acetate Extracts from Mangrove Plants Rhizophora apiculata and Sonneratia alba — Associated Fungi

R Dyana Sartika, Annisa Rahma, Salsabillah Amelano, Dian Handayani
2021 Proceedings of the Conference on Health and Wellbeing in Modern Society (CHW 2021)   unpublished
This study aims to explore the source of antibacterial compounds from mangrove endophytic fungi. Nine fungi were isolated from the leaves, barks, and roots of mangrove Rhizophora apiculata and Sonneratia alba from West Sumatera, Indonesia. The fungal isolate was cultivated in rice for 4-6 weeks, and subsequently, its secondary metabolites were extracted using ethyl acetate. The ethyl acetate extracts were tested against pathogenic bacteria; Bacillus cereus, Multi-Drug Resistant Pseudomonas
more » ... ginosa, Staphylococcus aureus, and Escherichia coli, and Mycobacterium tuberculosis. Seven extracts showed antibacterial activity at 5% (b/v %) concentration in the diffusion method. The highest inhibition was exhibited by the extract of RAA1 with the range of inhibition zone of 21.87 ± 0.81 mm -26.12 ± 0.21 mm. Six fungal extracts (RAA2, RAA3, RAA4, RAK2, SaDa1, SaDa2) showed sensitivity to M. tuberculosis at concentrations of 3% and 5%. The potential fungi were identified molecularly by DNA amplification and sequencing of the fungal ITS region. The results of identification showed RAA1 as Aspergillus flavus, RAA4 as Aspergillus aculeatus, and SaDa1 as Penicillium rubens.
doi:10.2991/ahsr.k.211105.049 fatcat:dzubrk2ngjazngfznfjmhkglc4