Public Health Virology, Forensic and Scientific Services This protocol was designed and developed at this laboratory. The assay targets the capsid peptide coding region of DENV 1-4 and is desigend as a qualitative screening test for human cases of DENV infection. Bioline Life Technologies If using a different brand or model of real-time thermocycler, check the concentration of ROX is adequate. Method assumes the user is familar with the thermocycler and software used to run the protocol.