Abstrac し Reflex bleeding is characteristic for a number of inseot spe じ ies such as Iadybeetles and leaf beetles . In thls study , we assessed the utility of re 刊ex bleeding as a noninvasive sQurce for DNA analysis . By using the method based on the nucleotide − acid binding property of silica particles , we were able to extract DNA from the re 伺ex bleeding exuded by some ladybeetles . In addition , using the extracted DNA , we were able to amplify and determine the nucleotide sequences く〕 f

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... S regi {m . These results suggest that the reflex bleeding will be a useful source for analyzing DNA in ln5ects without killing them . Key words : reflex bleeding , DNA extraction , PCR , DNA sequencing