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Construction and Application of a Family-specific Primer for the Detection;of Gramineous Raw Materials in Foods
food preservation science
We constructed a gramineous plant-specific primer set-matK 7 9 2F1GC and matK 9 7 9R1, targeting the maturase K(matK )gene, for detection of gramineous raw materials in processed food. For this, we used polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) . The primer set was evaluated using agarose gel electrophoresis and DGGE. Our results confirmed that the primer set was specific for gramineous plants. From our analysis, we confirmed that up to 6 types of gramineousdoi:10.5891/jafps.40.285 fatcat:em4iheberna5ng2lb6d24hkiri