CYTOPHOTOMETRIC ESTIMATION OF 4C DNA CONTENT AND KARYOTYPE ANALYSIS IN TEN CULTIVARS OF TRIGONELLA FOENUM-GRAECUM

A Das, S Mohanty, P Das Das, S Das
unpublished
, P. 2001 12 15: Cytophotometric estimation of 4c DNA content and karyotype analysis in ten cultivars of Trigonella foenum-graecum.-Iran. Journ. Bot. 9 (I): 1-9. Tehran. Estimation of nuclear DNA content through cytophoto-densitorneter in somatic cells and karyotype analysis were carried out for the first time in ten cultivars of fenugreek (Trigonella foenum-graecum). Somatic chromosome number is reported 2n=16 in all the cultivars. Detailed karyotype analysis revealed cultivar specific
more » ... ar specific chromosomal characteristics and minute structural alterations in chromosomes. The 4C DNA content varied significantly from 7.23 pg in the cultivar TO-I03 to 9.12 pg in TO-09. Significant variations in the genome length, volume and total form percentage were noted at cultivar level. A positive significant correlation coefficient revealed interdependence between the chromosome volume and nuclear DNA content of the cultivars. Significant variations in 4C nuclear DNA content among different cultivars despite the same somatic chromosome number suggest a changes in the repetitive DNA sequences of the genomes. A. B. Dos, S. Mohanty and P. Das, Cytogenetics Laboratory, Regional Plant Resource Centre, Bhubaneswar 751 ()I 5, Orissa, India
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