A Rapid and Sensitive Single Residual Method for Determination of Ethephon in Grape by high performance liquid chromatography-tandem mass spectrometry

Anna Luíza Brito Souza, Jessyca Wanessa Soares Araújo Silva, Henrique Carlos Marinho Pereira Silva, Analúcia Guedes Silveira Cabral, Clayton Anderson Azevedo Filho, Ellison Neves Lima, Carlos Eduardo Miranda Sousa
2019 Brazilian Journal of Health Review  
This paper describes a rapid (7.0 min) and sensitive (LLOQ 0.1 ng/mL) analytical method for the quantitation of Ethephon in grape. A new method for the detection and quantification of ETP residues in fruit and vegetables was developed. The present study indicates that fruit and vegetables require a rapid and simple cleanup step before using gas chromatograph/mass spectrometry. The recovery and precision of the new method were evaluated by spiking the fruit and vegetable samples with 0.01-0.1
more » ... es with 0.01-0.1 μg/g of ETP. The method is based on High-performance Liquid chromatography-tandem mass spectrometry (LC-MS/MS).The chromatographic separation was achieved on a Hypercarb 2.1x 100 mm 5µm reversed-phase column and a mobile phase containing water/mathanol (97:3 v/v, add 1% acetic acid), in isocratic conditions. The target analytes were transferred into a triple quadrupole mass spectrometer equipped with an electrospray ionization source for mass detection. The ion transitions selected for MRM detection were: m/z 142.8 >106.8, m/z 144.8 >106.8 and 106.8>78.8 for ETP. The linearity of the detector response was demonstrated in the range from 0.01 to 1 µg/g for each analyte with a coefficient of determination (R 2 ) of ≥0.999. The method was successfully applied to determination of ethephon in bunch grapes from five free trade fairs in the city of Caruaru, Pernambuco, Brazil.
doi:10.34119/bjhrv2n6-005 fatcat:353aqcvzvfhqjm3lqoynq4v7hi