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Design and optimisation of hammerhead ribozymes
2022
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CONTENTS CHAPTER 1 2.3.1 Preparation of double stranded template DNA for sequencing 2.3.2 Double stranded DNA reactions 2.4 RNA synthesis 2.4.1 32P-labelled RNA 2.4.2 Capped RNA 2.5 TCA precipitation of RNA 2.6 RNA extraction from polyacrylamide gels 2.7 In vitro ribozyme reactions 2.8 Preparation of E. coli for electro-transformation 2.9 Electro-transformation of E. coli. 2.10 Dot blotting 2.10.1 Low stringency wash 2.10.2 Medium stringency wash 2.10.3 High stringency wash 2.11 Isolation of
doi:10.17638/03146636
fatcat:jnc54ceimjaw3ocqkxuld32pdm