Noncationic Peptides Obtained From Azurin Preferentially Enter Cancer Cells

B. N. Taylor, R. R. Mehta, T. Yamada, F. Lekmine, K. Christov, A. M. Chakrabarty, A. Green, L. Bratescu, A. Shilkaitis, C. W. Beattie, T. K. Das Gupta
2009 Cancer Research  
Azurin, a member of the cupredoxin family of copper containing redox proteins, preferentially penetrates human cancer cells and exerts cytostatic and cytotoxic (apoptotic) effects with no apparent activity on normal cells. Amino acids 50 to 77 (p28) of azurin seem responsible for cellular penetration and at least part of the antiproliferative, proapoptotic activity of azurin against a number of solid tumor cell lines. We show by confocal microscopy and fluorescence-activated cell sorting that
more » ... cell sorting that amino acids 50 to 67 (p18) are a minimal motif (protein transduction domain) responsible for the preferential entry of azurin into human cancer cells.
doi:10.1158/0008-5472.can-08-2932 pmid:19147567 fatcat:siyun2xfsbasfi7pkwu62wjxee