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The Optimized Conditions of Two Dimensional Polyacrylamide Gel Electrophoresis for Serum Proteomics
2008
Journal of Proteomics & Bioinformatics
Serum proteome analysis provides a potential promising approach in disease diagnosis and therapeutic monitoring. However, the large dynamic range of proteins, high-abundant proteins, excess of salt and lipid in serum makes the analysis very challenging. Therefore, it is imperative to improve the dissolution of proteins in twodimensional gel electrophoresis (2-DE), and enhance the ability to analyze the proteome of serum under a wide variety of physiological conditions. This study examined the
doi:10.4172/jpb.1000032
fatcat:hfhpcmulwzhkpnvjn5smbtbewa