Open defaecation leads to faecal matter contamination of drinking water which can cause water borne diseases. The study assessed the linkage between sanitation practices and microbial quality of drinking water of open defaecation free and open defaecation communities in the Savelugu Municipality. A cross-sectional survey of 170 households was conducted in five open defaecation free and five open defaecation communities in the Savelugu Municipality. A total of 78 samples were collected from

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... sources and households and analysed for Escherichia coli, Salmonella spp and Shigella spp. Sanitation facilities used by the residents included pit latrine (88.24%), no latrine (11.17%), and flush (0.59%). The study observed improper disposal of children faeces in the communities. E. coli count ranged from 0 to 15 CFU/100 ml in open defaecation free communities and 0 to 32 CFU/100 ml in open defaecation communities. The microbial load in the drinking water from open defaecation communities was higher than open defaecation free communities. Open defaecation practice leads to contamination of household water probably due to unsanitary. Community Led Total Sanitation (CLTS) should be encouraged in open defaecation communities.