The present study aimed to evaluate the incidence and seasonal distribution of Staphylococcus species on a medium-scale dairy farm and investigate their antimicrobial sensitivity with selected standard antibiotics. Several biochemical tests with the API staph kits were utilized to recognize the presence of micro-bacteria in the milk a species level. The antimicrobial activity was evaluated following the approved Clinical Laboratory Standard Institute (CLSI) guidelines. In total, 217 samples of

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... ilk were cultured for bacterial tests over nine months. Of the 217 milk samples analyzed, 13 varied Staphylococcus species were identified from the 86 (39.63%) positive isolates. Of the identified isolates, Staphylococcus xylosus (22.09%), Staphylococcus hominis (15.11%), and Staphylococcus haemolyticus (11.63%) were the most common. Bacteria species that showed resistance to the 15 different antimicrobial agents used in this study included Staphylococcus xylosus (80%), Staphylococcus sciuri (60%), Staphylococcus aureus (53.33%), Staphylococcus haemolyticus (46.67%), Staphylococcus epidermidis (46.67%), and Staphylococcus chromogenes (40%). The results found a high incident rate and multiple Staphylococcus species resistant to antimicrobial drugs like penicillin and ampicillin, which are utilized on the farm, which calls for drastic measures.