It has been reported, in our previous paper, that urine proteins were fractionated into 21 bands on sodium dodecyl sulfate polyacrylamide gel. When the precipitate from antigen antibody reaction was used as a standard, proteins in 7 major bands were assumed with a fairly high probability . 21 bands were numbered from 1 to 21 from the cathode side, band 2 was α2-macroglobulin , band g was 2H+L chain, band 10 was transferrin, band 11 was albumin, band 13 was H chain , band 17 was L chain. and

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... 20 was β2-microglobulin. Six bands out of the 8 bands which may be obtained from normal urine were found to correspond to 6 bands out of the 7 bands identified. If it is assumed that each of these major bands contains a single protein, the percentage of transferrin clearance which reflects the severity of glomerulonephritis may be estimated by the present method.