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A simple, sensitive, selective rapid spectrophotometric method has been developed for the determination of post synaptic α 1-Adrino receptor antagonist Methotrexate in pure form and pharmaceutical formulations based on the oxidative coupling reaction with MBTH reagent, at P H_ 4.0 which is extractable at 610 nm. Beer's law is obeyed in the concentration ranges 5-30 µg ml-1. The developed method was applied directly and easily for the analysis of the Pharmaceutical formulations. R.S.D was foundfatcat:5ddh7ql5jbaw3c5zjfoajmsahm