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Uncoating of human rhinovirus serotype 2 from late endosomes

E Prchla, E Kuechler, D Blaas, R Fuchs
1994 Journal of Virology  

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The internalization pathway and mechanism of uncoating of human rhinovirus serotype 2 (HRV2), a minor-group human rhinovirus, were investigated. Kinetic analysis revealed a late endosomal compartment as the site of capsid modification from D to C antigenicity. The conformational change as well as the infection was prevented by the specific V-ATPase inhibitor bafilomycin Al. A requirement for ATP was also demonstrated with purified endosomes in vitro. Capsid modifications occurred at a pH of 5.5
more » ... regardless of whether the virus was entrapped in isolated endosomes or free in solution. These findings suggest that the receptor is not directly involved * Corresponding author. Mailing address:
doi:10.1128/jvi.68.6.3713-3723.1994 fatcat:crobvmjqyra43md6pcysitirva
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E Prchla, E Kuechler, D Blaas, R Fuchs. "Uncoating of human rhinovirus serotype 2 from late endosomes." Journal of Virology 68.6 (1994) 3713-3723