As suggested by the reviewers of our grant the cyclopropylquinone, similar to the time aPl:;lication_ most of our efforts have focused on the dependence for the in vivo inhibition (2,3), ,=, first'set of our primary objectives. We have pursued suggestil_ a rather high turnover number before the inhibitor development and found that the inhibitors inhibitorleads to enzyme inactivation. Proceedingon (both the cyclopropyl-and oxiranequinones) are that assumption, the product distributionof the [3H]

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... intolerant of other substituents on the quinone cyclopropylcompound following incubationwith the nucleusand are only marginallytolerant to substitution lower phase membrane fraction was monitoredwith on the three-membered rings. Therefore the HPLC. A proposal to explain the productsthat w_,re production of labelled compounds have been found is given in Scheme !. The dominantreaction is the reversible addition and removal of one electron restricted to 3H and 14C isotopes. The tritiated from the quinone. The subsequent mechanisms cyclopropane and oxirane benzoquinones were ., ,....