Mantenimiento y titulación del virus de dengue para obtención de antígeno viral

Carmen Roig, V Aquino, Ivalena Guillén, Alejandra Rojas, Estela Picaguá, Maria Eugenia Acosta, Laura Aria, Teresa Meza, Eva Nara
2020 Memorias del Instituto de Investigaciones en Ciencias de la Salud  
Dengue is a serious public health problem that has neither vaccine nor specific treatment. Serology is the most frequently used diagnosis method, specifically the anti-dengue IgM detection. The viral antigens employed in this method could be prepared from Aedes albopictus cell cultures (C6/36). The objective of this study was to maintain the four viral serotypes [D1 (RIO), D2 (RIO), D3 (H-87), D4 (BV)] on C6/36 cells for the preparation of viral antigen in the future. The C6/36 cells were
more » ... ed in L-15 medium supplemented with 10% FCS, infected with 50 µl of each viral serotype and then incubated for 5-7 days at 28° C. After confirmation of the infection by indirect immunofluorescence (IIF), viral titration was performed by lysis plaque assay. The serotypes titres obtained were as follows: [2.9 x 10 6 PFU/ml] for D1 (RIO), [4.4 x10 7 PFU/ml] for D2 (RIO), [6.4 x 10 7 PFU/ml] for D3 (H87) and [5.1 x10 6 PFU/ml] for D4 (BV). The production of viral antigens is very important because they could be used in several diagnosis methods.
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