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Efficient Protocol for Protoplast Isolation and Plant Regeneration of Fritillaria imperialis L
J. Agr. Sci. Tech
The present study reports an efficient protocol for isolation and regeneration of protoplasts from callus of Fritillaria imperialis L. There is no published method recommended for protoplast isolation and regeneration from Fritillaria imperialis L. A range of factors, which influence the success of isolation and regeneration of F. imperialis protoplasts, were investigated. From the results obtained, callus Fresh Weight (FW) of 0.4 g produced the highest number of viable protoplasts, which wasfatcat:v347wc7e2ja3reh4ne2ujlzwwi