To apply Haematoxylin and Eosin stain and grading the gastrointestinal lymphomas, To assess the applicability of AGNOR stain in gastrointestinal lymphomas and for discrimination of high and low grade lymphoma. 21 cases from Institute of Pathology and Electron microscopy, Madras Medical College and Institute of Child Health, Egmore, Chennai were studied.These cases were diagnosed and sub typed with the help of H and E stain. For AgNOR staining, tissues fixed in 10% formalin processed to paraffin

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... wax were cut at three microns thickness and stained. In our study 7 cases out of 21 were NHL-low grade and the mean AgNOR value is 14.6. Remaining 14 cases show high grade lymphoma and their mean AgNOR value is 55.5. In our study 3.3 is used as cut of point to discriminate between low and high grade lymphomas. Being a simple technique, easy to perform, AgNOR can be used as a routeine stain for discriminating high and low grade lymphoma.