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Stimulated Raman scattering (SRS) microscopy is a labelfree chemical imaging technique. Two-color imaging is often necessary to determine the distribution of chemical species in SRS microscopy. Current multi-color SRS imaging methods involve complicated instrumentation or longer data acquisition time or are limited to transmission imaging. In this Letter, we show that by adding a simple fiber amplifier to a 2 ps laser source and optical-parametric-oscillatorbased SRS setup, one can achievedoi:10.1364/ol.42.000523 pmid:28146518 fatcat:7tgnq45bnfcgldnoh22utmgk54